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Figure 7 | BMC Biochemistry

Figure 7

From: Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin and regulates the phosphorylation and activation of the mammalian Ste20-Like kinase Mst3

Figure 7

Loss of striatin residues 227-309 nearly abolishes Mst3 binding while loss of the calmodulin domain greatly enhances the binding of both Mst3 and Mst4. (A) Three days after transfection with HA-tagged wild-type and mutant striatins, HEK293 cells were lysed and HA-striatin immune complexes were isolated, separated by SDS-PAGE, and immunoblotted. The Mob3 panel shown has been compressed 25% in the vertical dimension to save space. (B) Relative binding of wild-type and mutant striatins to Mst3, Mst4, and Mob3 was measured as described in the legend to Figure 2B. The error bars represent the standard error of four independent experiments. *, p ≤ 0.05; **, p ≤ 0.01 relative to wild-type.

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