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Figure 6 | BMC Biochemistry

Figure 6

From: Protein phosphatase 2a (PP2A) binds within the oligomerization domain of striatin and regulates the phosphorylation and activation of the mammalian Ste20-Like kinase Mst3

Figure 6

CCM3 binding to striatin is affected by the same deletions that reduce Mst3 binding. (A) Three days after transfection with HA-tagged wild-type and mutant striatins, HEK293 cells stably expressing FLAG-CCM3 were lysed and FLAG-CCM3 immune complexes were isolated, separated by SDS-PAGE, and immunoblotted. Lysate levels of the transfected HA-tagged striatins are shown for comparison to the amount of HA-striatins that coimmunoprecipitated with FLAG-CCM3. (B) Relative binding of wild-type and mutant striatins to FLAG-CCM3 was measured as described in the legend to Figure 2B, except that in this experiment the values were normalized to expression of the HA-constructs in the cell lysates. The error bars represent the standard deviation of three independent experiments. **, p ≤ 0.01 relative to wild-type.

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