Figure 4

OGDHC activity regulated by nucleotide with and without calcium (A, B) OGDHC activity as a function of various concentrations of ATP (circle), ADP (up-triangle) or ADP with 1.5 mM ATP (down-triangle) in the presence of 1.0 mM NAD, 0.25 mM CoASH, with the addition of either 0.1 mM 2-oxoglurate plus 5 mM EGTA plus 5 mM CaCl2 (A) or 2 mM 2-oxoglurate plus 5 mM EGTA (B). Kinetic data were obtained from Figure Five of [23]. (C) OGDHC activity as a function of ADP/ATP ratio in the presence of 1.0 mM NAD, 0.25 mM CoASH and either 0.1 mM 2-oxoglurate plus 5 mM EGTA plus 5 mM CaCl₂ (circle) or 2 mM 2-oxoglurate plus 5 mM EGTA (up-triangle). Kinetic data were obtained from Figure Six of [23]. Solid lines are model fitting results for the data points represented by symbols.