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Table 1 Purification of PGC2 from culture of FOC4 grown on SM supplemented with 1% citrus pectin as the sole carbon source

From: Isolation and characterization of an exopolygalacturonase from Fusarium oxysporum f.sp. cubense race 1 and race 4

Step

Total protein

(mg)

Total activity

(Unit)a

Yield

(%)

Specific activity

(Units mg protein-1min-1)

Crude

36.60

131.25

100

3.59

Ultrafiltration

8.21

70.26

53.53

8.56

Sephacryl S-100 16/60

1.85

20.35

15.51

11

Sepharose FF CM Hitrap

0.26

5.54

4.22

21.30

  1. a One unit of PG activity was defined as the amount of enzyme that liberated one μmole of GA in one minute at 45°C.
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BMC Biochemistry

ISSN: 1471-2091

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