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Figure 5 | BMC Biochemistry

Figure 5

From: Isolation and characterization of an exopolygalacturonase from Fusarium oxysporum f.sp. cubense race 1 and race 4

Figure 5

SDS-PAGE analysis of recombinant PGC2 from FOC1 and FOC4 produced in P. pastoris. (a) Cultures were induced for 3 d with methanol as described in Materials and Methods and supernatants from 1, 2, and 3 d were collected. Lane M: protein marker. Lane 1: cultures transformed with pPICZaA. Lane 2-4: culture supernatant from FOC1 at 1, 2, and 3 d. Lane 5-7: culture supernatant from FOC4 at 1, 2, and 3 d. (b) SDS-PAGE analysis of purified PGC2 from FOC1 and FOC4 produced in P. pastoris. Lane M: protein marker. Lane 1: cultures transformed with pPICZaA. Lane 2: purified r-FOC1-PGC2. Lane 3: purified r-FOC4-PGC2.

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BMC Biochemistry

ISSN: 1471-2091

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