Figure 6

ChIP analysis of of the BRCA1 promoter. HeLa cells were crosslinked with 1% formaldehyde. Chromatin was then isolated and sonicated into approximately 1 kb fragments followed by immunoprecipitation with either control antibody (IgG), α53BP1 or αSRC3 antibody (IP) as indicated. The recovered DNA was then assayed by (A) conventional PCR or by (B) realtime PCR using pairs of oligonucleotides contained within the BRCA1 upstream region.