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Figure 4 | BMC Biochemistry

Figure 4

From: Regulation of the BRCA1 gene by an SRC3/53BP1 complex

Figure 4

Localization of 53BP1 and SRC3 in response to DNA damage. (A) HeLa cells were treated with etoposide for 30 min followed by fixation and immunoflourescence analysis using either α53BP1 or αSRC3 primary antibodies. (B) HeLa cells were treated with 20 Gy γ-irradiation and allowed to recover for 30 min prior to staining with α53BP1 polyclonal antibody directly labelled with Alexafluor 594 or αSRC3 polyclonal antibody labelled with Alexaflour 488. DAPI, DAPI fluorescence marking the cell nuclei.

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