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Figure 4 | BMC Biochemistry

Figure 4

From: Factors that influence the response of the LysR type transcriptional regulators to aromatic compounds

Figure 4

The inducer response in E.coli DH5α for DntR (black bars), NagR (light grey bars) and NtdR (dark grey bars) for three experimental conditions. (a) E.coli DH5α harbouring the two-plasmid system for DntR, NagR and NtdR grown in LB. (b) E.coli DH5α harbouring the one-plasmid system for DntR, NagR and NtdR grown in LB (c) E.coli DH5α harbouring the one-plasmid system for DntR, NagR and NtdR grown in modified M9 medium. For all conditions the measurements were made 15 hours after addition of the potential inducers. Shown in the diagrams is the increase in fluorescence after addition of the potential inducer, compared to the control culture where only the solvent DMSO (or DMSO+IPTG in A) was added. The mean fluorescence was measured for 10 000 cells in each measurement. All data are based on three independent analyses and shown in error bars are the standard deviations based on these measurements.

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