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Table 2 Protease activity analysis using the natural substrates serum albumin and hemoglobin

From: In vitro activation and enzyme kinetic analysis of recombinant midgut serine proteases from the Dengue vector mosquito Aedes aegypti

Protease

BSA Digestion

Rate a

BSA Protease

Activity b

Hb Digestion

Rate a

HB Protease

Activity b

AaET

0.253 ± 0.027

42

1.66 ± 0.08

55

AaSPVI

0.341 ± 0.008

57

5.34 ± 0.43

177

AaSPVII

0.247 ± 0.014

41

1.60 ± 0.08

52

AaLT

0.294 ± 0.003

47

1.61 ± 0.15

52

Bovine Trypsin

0.291 ± 0.016

49

5.53 ± 0.12

186

  1. aUnits of nM min-1
  2. bUnits of nmol min-1 (mg protein)-1
  3. Rates were determined from plots of amount of BSA or Hb (nM) over time (min).