Figure 4

Representative gels of albumin (BSA) and hemoglobin (Hb) digestion by activated proteases for use in quantitating enzyme activities using natural blood protein substrates. The doublet in the Hb digestion reactions corresponds to the α and β subunits, which were both present in the commercial Hb preparation. For these experiments, all five enzymes were used at the same mass ratios, which were 922:1 for BSA:protease and 214:1 for Hb:protease. Rates of digestion from similar digestion reactions were determined using image analysis quantitation as described in Materials and Methods. Digestion data from multiple reactions run in triplicate are presented in Table 2.