Figure 3

Partial proteolysis of BSA using BvT or the activated forms of each mosquito enzyme. A) Partial proteolysis of BSA in the absence (Cntrl) or presence of BvT for 15 or 60 min. The reactions were terminated with SDS-PAGE loading buffer and boiling for 3 min. The gel is stained with GelCode Blue reagent. B) Partial proteolysis products of BSA generated by in vitro cleavage reactions using each of the indicated enzymes for 15 or 60 minutes. The mass ratio of BSA:protease was 10:1 for all reactions except for the AaSPVII reaction, in which the mass ratio of BSA:protease was 298:1 owing to the high BSA cleavage activity of this enzyme relative to the others.