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Figure 5 | BMC Biochemistry

Figure 5

From: The testis-specific Cα2 subunit of PKA is kinetically indistinguishable from the common Cα1 subunit of PKA

Figure 5

Phosphotransferase activity of Cα2 is inhibited by the R subunits and PKI in dose-dependent manner. Recombinant Cα2 (30 nM) mixed with Kemptide (250 μM) and ATP (10 mM) was incubated in the presence of increasing concentrations (0 - 25 nM) of either RIα and RIβ (panel A) and RIIα and RIIβ (panel B). Cα2 is inhibited by all R subunits in a dose-dependent manner at equimolar concentrations of RIα, RIβ, RIIα and RIIβ. Panel C: The inhibitory effect of PKIα was verified by incubating recombinant Cα2 (28 nM) in the presence of Kemptide (250 μM), ATP (10 mM) and increasing concentrations of recombinant PKIα (0-40 nM). Titration curves shown are normalized before linear regression (n = 2).

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