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Figure 7 | BMC Biochemistry

Figure 7

From: SUMO-1 regulates the conformational dynamics of Thymine-DNA Glycosylase regulatory domain and competes with its DNA binding activity

Figure 7

Competition between TDG-RD and SUMO-1 for DNA binding. 20 μM 15N-labeled TDG-N with an equimolar amount of a dsDNA-25mer substrate containing a G:T mismatch (blue spectrum) was submitted to a 4-fold molar excess of SUMO-1 (80 μM) (red spectrum). Spectra of isolated 15N-TDG-N at 20 μM alone (black spectrum) and in presence of 80 μM of the same DNA substrate (green spectrum) were given as references. A significant displacement of RD/DNA complex by SUMO-1 is observed for residues E75, K78, S82, S85, S88 and S91. Absence of competition is found for residues Q55, A57, K64 and E69 indicating a partial competition between SUMO-1 and TDG-RD centred on the region 75 to 91.

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