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Figure 5 | BMC Biochemistry

Figure 5

From: SUMO-1 regulates the conformational dynamics of Thymine-DNA Glycosylase regulatory domain and competes with its DNA binding activity

Figure 5

Glycosylase kinetics of TDG proteins on G:T (A) and G:U (B) repair. The curve of unmodified TDG (black square) is represented as broken lines for reference. The kinetics curves of sumoylated TDG (black square) and TDG-ΔN (black diamond) are represented by black lines. The kinetic curves of TDG in presence of 5 equivalents (red square) and 10 equivalents (blue square) of SUMO-1 are represented by dotted lines. (C, D) As in (A, B) using TDG-E310Q protein. (E, F) Graphical representation of the enzymatic turnover rates for the G:T (dark grey bars) and G:U (light grey bars) glycosylase activity of TDG (E) and TDG-E310Q (F). (G) Control of protein integrity in SDS-PAGE.

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BMC Biochemistry

ISSN: 1471-2091

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