Skip to main content
Figure 3 | BMC Biochemistry

Figure 3

From: SUMO-1 regulates the conformational dynamics of Thymine-DNA Glycosylase regulatory domain and competes with its DNA binding activity

Figure 3

(A) 15N-1H HSQC spectra of TDG alone (black) at 20 μM or in presence of 120 μM of SUMO-1 (red). Resonances of TDG-RD are annotated in bold and resonances of the C-terminal residues in italic. Some resonances of unlabeled SUMO-1 are detectable at 15N natural abundance. (B) 15N-1H HSQC spectra of 15N-TDG-E310Q at 20 μM without SUMO-1 (black) or in presence of 200 μM SUMO-1 (red). The 15N-1H HSQC of 15N-SUMO-1 (green) is shown as a reference to identify SUMO-1 resonances detected at 15N natural abundance. Resonances of the C-terminal domain are indicated in italic. Resonances of TDG-RD, indicated in bold, exhibiting a very low intensity on TDG and TDG-E310Q spectra appear with a higher intensity in presence of SUMO-1 for TDG only. (C) The overlay of the 15N-1H HSQC spectra of the 15N-TDG (black) and the isolated 15N-TDG-N (blue) identifies the resonances of TDG-RD residues.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us