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Table 2 Kinetic parameters of T. cruzi TS, T. congolense TS1b and TS1 e-1

From: Biochemical characterization of trans-sialidase TS1 variants from Trypanosoma congolense

  Acceptor substrates Donor substrates
  Lactose Gal-MU Fetuin-bound Siax
  vmax
(μmol/(min × mg TS))
KM
(μM)
vmax
(μmol/(min × mg TS))
KM
(μM)
vmax
(μmol/(min × mg TS))
KM
(μM)
TS1b 4.3 ± 0.1 1683 ± 101 0.77 ± 0.03 57 ± 14 7.9 ± 0.3 359 ± 45
TS1 e-1 2.1 ± 0.1 727 ± 48 0.72 ± 0.03 74 ± 17 7.6 ± 0.5 1617 ± 223
T. cruzi TS 8.4 ± 0.3 327 ± 31 n.d. n.d. 37.9 ± 6.0 4124 ± 985
  1. KM and vmax were calculated from Michaelis-Menten kinetics (Figure 7) by SigmaPlot. Data points are means ± standard deviations of three replicates. × Approximately 30 nmol Sia per 100 μg fetuin. Abbr.: n.d.: not determined.