Table 2 Kinetic parameters of T. cruzi TS, T. congolense TS1b and TS1 e-1
From: Biochemical characterization of trans-sialidase TS1 variants from Trypanosoma congolense
| Â | Acceptor substrates | Donor substrates | ||||
|---|---|---|---|---|---|---|
| Â | Lactose | Gal-MU | Fetuin-bound Siax | |||
|  | vmax (μmol/(min × mg TS)) | KM (μM) | vmax (μmol/(min × mg TS)) | KM (μM) | vmax (μmol/(min × mg TS)) | KM (μM) |
TS1b | 4.3 ± 0.1 | 1683 ± 101 | 0.77 ± 0.03 | 57 ± 14 | 7.9 ± 0.3 | 359 ± 45 |
TS1 e-1 | 2.1 ± 0.1 | 727 ± 48 | 0.72 ± 0.03 | 74 ± 17 | 7.6 ± 0.5 | 1617 ± 223 |
T. cruzi TS | 8.4 ± 0.3 | 327 ± 31 | n.d. | n.d. | 37.9 ± 6.0 | 4124 ± 985 |