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Table 1 Free Sia and 3'SL production at equilibrium of the reaction.

From: Biochemical characterization of trans-sialidase TS1 variants from Trypanosoma congolense

 

Neu5Ac [pmol]

3'SL [pmol]

0 ng TS

262 ± 46

0

50 ng T. cruzi TS

580 ± 8

12597 ± 115

250 ng T. cruzi TS

1820 ± 13

11199 ± 288

50 ng TS1b

415 ± 7

15378 ± 117

250 ng TS1b

1095 ± 129

14435 ± 2226

50 ng TS1 e-1

348 ± 83

12719 ± 3057

250 ng TS1 e-1

1186 ± 9

13655 ± 491

only fetuin

116 ± 8

0

  1. Neu5Ac and 3'SL produced by TS after 20 h incubation under standard conditions were determined as described under Material and Methods. Data points are means ± standard deviations of three replicates.
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BMC Biochemistry

ISSN: 1471-2091

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