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Table 1 Free Sia and 3'SL production at equilibrium of the reaction.

From: Biochemical characterization of trans-sialidase TS1 variants from Trypanosoma congolense

  Neu5Ac [pmol] 3'SL [pmol]
0 ng TS 262 ± 46 0
50 ng T. cruzi TS 580 ± 8 12597 ± 115
250 ng T. cruzi TS 1820 ± 13 11199 ± 288
50 ng TS1b 415 ± 7 15378 ± 117
250 ng TS1b 1095 ± 129 14435 ± 2226
50 ng TS1 e-1 348 ± 83 12719 ± 3057
250 ng TS1 e-1 1186 ± 9 13655 ± 491
only fetuin 116 ± 8 0
  1. Neu5Ac and 3'SL produced by TS after 20 h incubation under standard conditions were determined as described under Material and Methods. Data points are means ± standard deviations of three replicates.