Figure 9

Relative changes of the sialylation pattern of fetuin N -glycans. Shown are the sialylation patterns of the glycopeptides GP 127-141 with dibranched (GP 127-141 di) or tribranched (GP 127-141 tri) glycans and of the glycopeptide GP 54-85 with tribranched glycans (GP 54-85 tri) relative to the non-sialylated GP 1 -32. 100 μg fetuin was incubated with 50 ng TS1b, TS1 e-1 or T. cruzi TS and 100 nmol lactose for the times indicated, trypsinized and glycopeptides analyzed by MALDI-MS as described under Methods. Data points are averages ± standard deviations of three to ten replicates.