Figure 6From: Biochemical characterization of trans-sialidase TS1 variants from Trypanosoma congolenseConcentration dependence of TS reactions. Reactions of 100 μg fetuin with 100 nmol lactose were started with varying amounts of TS in overall 50 μL 10 mM phosphate buffer, pH 7.4 and incubated at 37°C for 30 min. The amount of 3'SL produced was determined by HPAEC-PAD as described under Methods. Data points are means ± standard deviations of three determinations.Back to article page