Figure 2

Processing of human plasminogen by GST-MMP-19. (A) 20 μg Glu-type plasminogen was either left undigested (lane 1) or was digested for 60 h by 5 μg MMP-9 (lane 2) which was activated before the digest with trypsin at 37°C for 20 min or 15 μg GST-MMP-19 (lane 3) at 37°C for 96 h. Digests were subjected to SDS-PAGE followed by Coomassie staining. Angiostatin-like fragments generated by MMP-19 are indicated by arrowheads. (B) Time course of human plasminogen processing by GST-MMP-19. 10 μg Plasminogen was digested by 8 μg GST-MMP-19 at 37°C for 96 h. Samples were analyzed at 0, 24, 48, 72, and 96 h on SDS-PAGE followed by silver staining. The mixture of digested plasminogen fragments was used without further purification in the tube-like formation assay.