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Figure 1 | BMC Biochemistry

Figure 1

From: Matrix metalloproteinase-19 inhibits growth of endothelial cells by generating angiostatin-like fragments from plasminogen

Figure 1

Purification and detection of recombinant GST-MMP-19 proteins. (A) 5 μg purified active MMP-19 protein (WT) and the mutant inactive form of MMP-19 harbouring the exchange of E213 to A (EA) were subjected to SDS-PAGE and stained with silver staining. (B) Purified MMP-19 WT and EA protein (5 μg) were detected by Western blotting using rabbit polyclonal antibodies (Dianova, dilution 1:3000). Arrowheads indicate MMP-19 at 85 kDa.

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