Figure 9
Effect of ATP, C8D-ATP and ATP/C8D-ATP (1:1 ratio) concentrations. Effect of ATP, C8D-ATP and ATP/C8D-ATP (1:1 ratio) concentrations on the specific activity and KIE of shikimate kinase (A), hexokinase (B), acetate kinase (C) and PFK (D). The theoretical activity was obtained using the constants and the best model defining each enzyme activity profile for ATP and C8D-ATP to estimate the activity at each concentration assuming mix of ATP/C8D-ATP. The activity estimated for ATP and C8D-ATP was summed to determine the activity for ATP/C8D-ATP. "black circle " = ATP, black square" = C8D-ATP, "black triangle" = ATP/C8D-ATP, Red = Theoretical activity, Blue = KIED. The specific enzyme activity is expressed as moles ADP formed per minute per milligram protein.