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Figure 3 | BMC Biochemistry

Figure 3

From: Amino-terminal extension present in the methionine aminopeptidase type 1c of Mycobacterium tuberculosis is indispensible for its activity

Figure 3

N-terminal deletion of Mt MetAP1c affects its enzyme activity. (A) Deletion scheme. Notations used: WT, wild-type; Δ, deleted residues. (B) Assessment of methionine aminopeptidase activity of deletion variants. Methionine aminopeptidase activity assay was performed using indicated amount of wild-type and mutant proteins with 4 mM of Met-Ala-Ser as the substrate. (C) Coomassie stained SDS-PAGE gel (upper panel) and Western blot (lower panel) using anti- His antibody of the mutant proteins as shown in Figure 3B. (D) Far-UV CD spectra of the WT, Δ2-10 and Δ2-15 proteins. (E) Near-UV CD spectra of the WT, Δ2-10 and Δ2-15 proteins.

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BMC Biochemistry

ISSN: 1471-2091

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