Nano-electrospray tandem mass spectrometric analysis of the acetylation state of histones H3 and H4 in stationary phase in Saccharomyces cerevisiae

Table 5 Fragment ions of the ²⁸SAPSTGGVKKPHR⁴⁰ peptide from histone H3 isolated from exponential phase Saccharomyces cerevisiae.

Order	b ion (m/z)	b²⁺ ion (m/z)	Sequence	y ion (m/z)	y²⁺ ion (m/z)	Order
1	88.0393	44.5233	S			13
2	159.0764	80.0418	A	1276.7120	638.8597	12
3	256.1292	128.5682	P	1205.6749	603.3411*	11
4	343.1612	172.0842	S	1108.6222	554.8147*	10
5	444.2089	222.6081	T	1021.5901	511.2987*	9
6	501.2304	251.1188	G	920.5425	460.7749*	8
7	558.2518	279.6295	G	863.5210	432.2641	7
8	657.3202	329.1638	V	806.4995	403.7534*	6
9	827.4258	414.2165	K	707.4311	354.2192	5
10	955.5207	478.2640	K	537.3256*	269.1664	4
11	1052.5735	526.7904	P	409.2306*	205.1190	3
12	1189.6324	595.3198*	H	312.1779	156.5926	2
13			R	175.1190	88.0631	1

The list of product ions observed (underlined), matched with Mascot to the SWISS-PROT database (asterisk superscript), or predicted following CID fragmentation is shown.

ISSN: 1471-2091