Figure 7

Western analysis of the acetylation state of histones H3 and H4. Equal amounts of core histones, isolated from exponential (Exp) and stationary (Stat) phase yeast cells, were resolved by SDS-PAGE, and a Western analysis performed using antibodies directed at acetylated lysine residues H3K9, H3K14, H4K12 and H4K16. A representative Coomassie-stained gel, demonstrating comparable histone H3 and H4 loading, is shown in the bottom panel, with the location of recombinant Xenopus H3 and H4 indicated in the left lane. The normalized volume of each band is indicated. The western blot band intensities were normalized by calculating the ratio to the intensity of the corresponding Coomassie-stained histone band, and this ratio was expressed as a percentage of the sum of the ratios for the exponential and stationary phase samples.