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Figure 1 | BMC Biochemistry

Figure 1

From: Nano-electrospray tandem mass spectrometric analysis of the acetylation state of histones H3 and H4 in stationary phase in Saccharomyces cerevisiae

Figure 1

K12 and K16 of histone H4 are acetylated in exponential phase. A tryptic digest of histone H4 isolated from exponential phase yeast cells was analyzed by MS. The enhanced precursor ion spectrum (A) and CID fragmentation spectrum of the peptide that resolved at m/z 464.4 ([M+2H]2+), indicated by the asterisk in panel A, is shown (B). The partial y-ion and b-ion series visible in the fragmentation spectrum are indicated, and the y6 and b8 peaks, representing terminal lysine residues shifted by an additional 42 Da, indicated by asterisks. The full m/z list of observed and predicted fragments are given in Table 1.

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BMC Biochemistry

ISSN: 1471-2091

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