Skip to main content
Figure 1 | BMC Biochemistry

Figure 1

From: All Dact (Dapper/Frodo) scaffold proteins dimerize and exhibit conserved interactions with Vangl, Dvl, and serine/threonine kinases

Figure 1

All Dacts run at higher than expected molecular weight and are phosphorylated in vivo. Lysates from HEK293 cells transiently transfected with plasmids expressing N-terminal FLAG-tagged murine Dact proteins, enzymatically treated as indicated, separated by SDS-PAGE, and visualized by immunoblotting with anti-FLAG antibody. All panels: lanes 1 & 3 untreated, lane 2 treated with phosphatase, Lane 4 treated with glycosylase. A, Dact1. B, Dact2. C, Dact3. In this and all subsequent figures, the number of replicate experiments is indicated in grey beneath each corresponding panel.

Back to article page

BMC Biochemistry

ISSN: 1471-2091

Contact us