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Table 1 Quantitation of Ixosc Sult 1 (R) and 2 (R) activity.a

From: Molecular characterization of novel sulfotransferases from the tick, Ixodes scapularis

Reaction mixture

Rate of reaction (nmol min-1mg-1)

PAP35S + Sult 1 (R) (Control)

0.00

p- Nitrophenol + Sult 1 (R) + PAP35S

1.30 ± 0.01

Dopamine + Sult 1(R) + PAP35S

1.52 ± 0.01

Octopamine + Sult 1(R) + PAP35S

1.34 ± 0.01

PAP35S + Sult 2 (R) (Control)

0.00

p- Nitrophenol + Sult 2 (R) + PAP35S

0.43 ± 0.01

Dopamine + Sult 2 (R) + PAP35S

0.39 ± 0.01

Octopamine + Sult 2 (R) + PAP35S

0.35 ± 0.01

  1. a Substrates (10 μM) were incubated with PAP35S (4 μM) and purified Ixosc Sult 1 (R)(0.04 mg/mL) or Ixosc Sult 2 (R)(0.03 mg/mL) for 30 min. Product formation in the absence of enzyme (PAP35S + substrate) was found to be zero.
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BMC Biochemistry

ISSN: 1471-2091

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