Figure 3
From: Molecular characterization of novel sulfotransferases from the tick, Ixodes scapularis
Analysis of Ixosc Sult 1 protein expression during feeding. (A) Ixosc Sult 1 detected by Western blot, (B) Quantitation of band intensity. Tissue homogenates from different developmental stages were analyzed by Western blot using anti-Sult 1 (R) raised antibody. Lanes: 1. Magic Marker, 2. Unfed larvae, 3. Fed larvae, 4. Unfed nymph, 5. Fed nymph. SULT 1 gene products were quantified using Kodak Digital Science 1D image analysis software and the net intensity/band intensity was plotted against the different tick stages.