Table 1 Thermodynamic parameters associated with binding of cysteine and methionine to OASS
Cysteine | Methionine | |||||
|---|---|---|---|---|---|---|
Temperature | ΔG (kJ/mol) | ΔH (kJ/mol) | TdS (kJ/mol) | ΔG (kJ/mol) | ΔH (kJ/mol) | TdS (kJ/mol) |
St OASS binding | ||||||
10°C | -9.6 ± 0.4 | -2.7 ± 0.1 | 6.9 | -8.6 ± 0.8 | -1.3 ± 0.1 | 7.3 |
15°C | -9.6 ± 0.3 | -2.8 ± 0.1 | 6.8 | -8.6 ± 0.4 | -1.4 ± 0.1 | 7.2 |
20°C | -9.8 ± 0.1 | -6.4 ± 0.1 | 3.4 | -9.7 ± 0.5 | -1.5 ± 0.1 | 8.2 |
25°C | -10.6 ± 0.1 | -5.9 ± 0.1 | 4.7 | -9.5 ± 0.5 | -0.9 ± 0.1 | 8.6 |
30°C | -10.2 ± 0.2 | -4.4 ± 0.2 | 5.8 | -10.4 ± 0.8 | -1.0 ± 0.1 | 9.4 |
35°C | -11.0 ± 0.2 | -5.4 ± 0.3 | 5.6 | -11.3 ± 1.3 | -1.2 ± 0.1 | 10.1 |
Hi OASSbinding | ||||||
10°C | -8.3 ± 0.4 | -4.6 ± 0.1 | 3.7 | -7.2 ± 0.9 | -0.2 ± 0.1 | 7.1 |
15°C | -8.7 ± 0.2 | -4.6 ± 0.2 | 4.1 | -7.2 ± 0.9 | -0.2 ± 0.1 | 7.5 |
20°C | -9.5 ± 0.5 | -4.4 ± 0.2 | 5.1 | -8.2 ± 1.0 | -0.2 ± 0.1 | 8.0 |
25°C | -9.5 ± 0.5 | -5.4 ± 0.3 | 4.1 | -7.6 ± 1.1 | -0.8 ± 0.1 | 6.9 |
30°C | -11.0 ± 0.3 | -4.5 ± 0.4 | 6.5 | -8.4 ± 1.0 | -0.7 ± 0.1 | 7.7 |