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Figure 5 | BMC Biochemistry

Figure 5

From: Comparative thermodynamic studies on substrate and product binding of O-Acetylserine Sulfhydrylase reveals two different ligand recognition modes

Figure 5

Temperature dependence of thermodynamic parameters determined from ITC experiments. (A) van't Hoff plot-temperature dependence of binding constants for cysteine and methionine binding to OASS; (closed circle) St OASS-methionine; (open circle) St OASS-cysteine; (B) Temperature dependence of binding enthalpy; (closed square) St OASS-cysteine; (closed circle) Hi OASS-cysteine; (open square) St OASS-methionine; (open circle-crossed) Hi OASS-methionine; horizontal line separates enthalpy for cysteine (Cys) binding from enthalpy contribution for methionine (Met) binding; (C) Temperature dependence of entropy; (open square) St OASS-methionine; (open circle) Hi OASS-methionine; (closed square) St OASS-cysteine; (closed circle) Hi OASS-cysteine; horizontal line separates net entropy change for cysteine (Cys) binding from entropic contribution for methionine (Met) binding;

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