Figure 4

ITC results mapped onto the crystal structure of E. coli HscB. Alanine substitution of L92, L96, or F153 (red) had the most detrimental effects on the affinity of HscB for apo-IscU, while alanine substitution at positions 87, 99, or 100 (green) resulted in smaller effects. Alanine substitution at positions 89, 93, 97, 103, 104, 152, 156, or 160 (dark grey) left the affinity of HscB for apo-IscU unchanged. This image was prepared with PyMol [30].