Figure 4From: Three hydrophobic amino acids in Escherichia coli HscB make the greatest contribution to the stability of the HscB-IscU complexITC results mapped onto the crystal structure of E. coli HscB. Alanine substitution of L92, L96, or F153 (red) had the most detrimental effects on the affinity of HscB for apo-IscU, while alanine substitution at positions 87, 99, or 100 (green) resulted in smaller effects. Alanine substitution at positions 89, 93, 97, 103, 104, 152, 156, or 160 (dark grey) left the affinity of HscB for apo-IscU unchanged. This image was prepared with PyMol [30].Back to article page