Figure 2From: Binding of ATP to vascular endothelial growth factor isoform VEGF-A165 is essential for inducing proliferation of human umbilical vein endothelial cellsEffect of increased ionic strength on labeling of VEGF-A 165 with [γ-32P]ATP and [α-32P]ATP. VEGF-A165 (3 μg) was incubated with radioactive ATP (5 μCi) in Tris-HCl (pH 7.5) at 37°C for 30 min. NaCl (100 mM) was added at times (t) indicated.Back to article page