Figure 8
From: Characterization of the expression, promoter activity and molecular architecture of fibin
Protein secretion studies. COS-7 cells transfected with (A,E) fibin, (B, F) myc-tagged fibin, (C, G) SEAP, and (D, H) GFP were treated with (A, B, C, D) 250 ng/ml Brefeldin A for 24 h or with (E, F, G, H) 50 ng/ml Brefeldin A for 6 h. Serum-free medium samples were collected and concentrated by trichloroacetic acid precipitation. Cell lysates and medium were analyzed by Western blot using the anti-fibin-2 antibody, anti-myc-POD antibody, anti-AP-antibody, anti-GFP antibody and anti-GAPDH antibody as well as the respective POD-labeled secondary antibodies.