Fibin mRNA expression estimated by qPCR. Total RNA was extracted either from several mouse tissues or cell lines and used for reverse transcriptase reaction. qPCR was carried out with transcript-specific primers pairs. (A) Fibin mRNA expression of tissues from three adult mice is given as x-fold over fibin expression in liver. Data are shown as means ± SEM from three different tissue and cell preparations. (B) At indicated days after fertilization embryos were dissected from uterus. Trunks, forelimbs and heads of day 10 and 11 were individual pools prepared from 20 mouse embryos, and therefore not marked with error bars. Data of day 13 were generated by three pools for each body part consisting of 3-5 individuals. For day 16 and 19 the body parts of three individuals were prepared independently. Data of days 13, 16, 19 are shown as means ± SEM. To compare fibin expression between embryonic and adult tissues qPCR was performed together with samples prepared from adult liver and cerebellum. (C) Fibin expression in human and murine cell lines is given as x-fold over expression levels in T98G cells and RAW264.7 cells, respectively. Data are shown as means ± SEM from three independent cell preparations. Significant differences are marked with *p < 0.05, **p < 0.01, ***p < 0.001 using the Student's unpaired t-test.