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Figure 5 | BMC Biochemistry

Figure 5

From: Role of redox environment on the oligomerization of higher molecular weight adiponectin

Figure 5

Re-oligomerization of hexameric adiponectin after treatment with βME.(A) Native PAGE of adiponectin oligomers after collapse of octadecamers to hexamers at pH 4 and subsequent neutralization in Tris-buffer and treatment with various concentrations of βME. (B) Non-reducing denaturing SDS-PAGE analysis of adiponectin dimers and monomers in reassembly reactions in part A. (C) Native PAGE analysis of adiponectin oligomers after collapse of octadecamers to hexamers at pH 4 and re-neutralization by dialysis against PBS followed by subsequent treatment with either 5 mM βME or PBS as control. (D) Non-reducing denaturing SDS-PAGE analysis of redox states of adiponectin oligomers in part C. Samples were removed and treated with NEM at the various time points to quench reactions.

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