Elucidating the domain architecture and functions of non-core RAG1: The capacity of a non-core zinc-binding domain to function in nuclear import and nucleic acid binding

Table 1 Zinc:Protein Ratio for CND and bZDD

RAG1 Domain	Conditions	Zinc:Protein Ratio ICP-MS^a,b	Zinc:Protein Ratio FAAS^a,b
CND^c	Zinc Free Buffer	2.08 ± 0.68	2.00 ± 0.44
CND^c	DTPA Buffer	0.24 ± 0.14	0.37 ± 0.29
bZDD^d	Zinc Free Buffer	4.23 ± 0.65	4.12 ± 0.78

a The zinc content of each construct was determined using ICP-MS and FAAS.
b Data presented represent the average of two or more independent experiments.
c Zinc binding results for mbp-CND and tagless CND were comparable. The results presented for CND represent the average from both data sets.
d Zinc binding for bZDD was evaluated for the mbp-tagged construct only.

ISSN: 1471-2091