Bioinformatic analysis of the N-terminal non-core region of RAG1. (A) Sequence conservation of the N-terminal non-core RAG1 region based on phylogenetic analysis. The z-score (y-axis) for each residue is a comparison of the observed conservation at that residue to the mean conservation across all sites in the sequence, and is the number of standard deviations away from the mean value . Positions with a more positive z-score are more highly conserved, whereas positions with a more negative score are less conserved. (B) Regions in the N-terminal non-core region of RAG1 predicted to be highly disordered are shown as thick bars. Each bar is labeled with N- and C-terminal residue numbers. Predictions 1 and 2 are results from DripPred and Disopred programs (see Methods), respectively. (C) The domain architecture of non-core RAG1 based on the combined experimental results (in Figure 2) and the bioinformatic methods.