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Table 1 Levels of 10A1 MLV and A-MLV entry supported by human PiT2 and derived truncation mutantsa.

From: Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life

Constructb No. (%) of cells infectedc
  A-MLV 10A1 MLV
  Expt 1 d Expt 2 d Expt 4 Expt 1 d Expt 2 d Expt 3 d Expt 4 Expt 5
PiT2 (pOJ74) 100 ±22 100 ± 8 100 ±12 100 ± 5 100 ± 8 100 ± 9 100 ±11 100 ± 9
PiT2ΔL183-V483 25 ± 1 13 ± 2 ND 10 ± 1 12 ± 4 24 ± 3 ND 25 ± 3
PiT2ΔR254-V483 NDe ND 4 ± >1 ND ND ND 1 ± >1 1 ± >1
Empty vectorf <0.0008 <0.002 <0.01 <0.002 <0.001 <0.001 <0.007 <0.003
  1. aThe experimental setup is described in the text. A-MLV and 10A1 MLV vector pseudotypes were tested on the same precipitates made from CsCl-purified plasmids in experiment 4. In experiment 5, Qiagen maxiprep-purified plasmids were used for preparing precipitates and only the 10A1 MLV vector pseudotype was tested.
  2. bReceptor and mutant receptor sequences were cloned into pcDNA1ARtkpA.
  3. cThe data are averages of three independent transfections ± SEM. The average number of blue cells per three 60-mm-diameter dishes transfected with a plasmid encoding PiT2 was assigned a value of 100% (57,000, 90,000, and 32,000 blue cells per dish for A-MLV in experiment 4 and for 10A1 MLV in experiments 4 and 5, respectively).
  4. dData are from Bøttger and Pedersen 2004 [31]; please see article for details.
  5. eND, not determined.
  6. fValues are based on the detection limit of 1 blue cell per three 60-mm-diameter dishes.