Mapping of the minimal inorganic phosphate transporting unit of human PiT2 suggests a structure universal to PiT-related proteins from all kingdoms of life

Table 1 Levels of 10A1 MLV and A-MLV entry supported by human PiT2 and derived truncation mutants^a.

^aThe experimental setup is described in the text. A-MLV and 10A1 MLV vector pseudotypes were tested on the same precipitates made from CsCl-purified plasmids in experiment 4. In experiment 5, Qiagen maxiprep-purified plasmids were used for preparing precipitates and only the 10A1 MLV vector pseudotype was tested.
^bReceptor and mutant receptor sequences were cloned into pcDNA1A^RtkpA.
^cThe data are averages of three independent transfections ± SEM. The average number of blue cells per three 60-mm-diameter dishes transfected with a plasmid encoding PiT2 was assigned a value of 100% (57,000, 90,000, and 32,000 blue cells per dish for A-MLV in experiment 4 and for 10A1 MLV in experiments 4 and 5, respectively).
^dData are from Bøttger and Pedersen 2004 [31]; please see article for details.
^eND, not determined.
^fValues are based on the detection limit of 1 blue cell per three 60-mm-diameter dishes.

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