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Figure 6 | BMC Biochemistry

Figure 6

From: Strand-specific PCR of UV radiation-damaged genomic DNA revealed an essential role of DNA-PKcs in the transcription-coupled repair

Figure 6

Transcription coupled repair (TCR) was detected by the strand-specific PCR, and depletion of DNA-PKcs led to decreased efficiency of TCR. A: A representative strand specific PCR to assay TCR in DNA-PKcs depleted HeLa-H1 and the control HeLa-NC cells. siRNA-mediated depression of DNA-PKcs resulted in a decreased repair efficiency of the DHFR gene transcribed-strand DNA damage induced by UV radiation, but there was no influence on the untranscribed strand DNA damage repair. B: Quantitative detection of TCR of the transcribed strand damage in DNA-PKcs depleted HeLa-H1 and control HeLa-NC cells. The DNA repair efficiency is expressed as the ratio of PCR products intensity of the DHFR transcribed-strand to β-actin. The data are the means of four independent experiments with standard deviation. * P < 0.05, # P < 0.01 as compared with HeLa-NC cells at the same time point. C: DNA repair (TCR) rate of the transcribed strand damage in DNA-PKcs depleted HeLa-H1 and control HeLa-NC cells after different time repair post UV irradiation. DNA repair rates were derived from the DNA repair efficiency data in Figure 6B, i.e. the ratio of PCR products intensity of the DHFR transcribed-strand to β-actin (ratio of TS to actin). DNA repair rate (%) = (Ratio of TS to actin at a repair time/untreated control) × 100

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