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Figure 2 | BMC Biochemistry

Figure 2

From: Strand-specific PCR of UV radiation-damaged genomic DNA revealed an essential role of DNA-PKcs in the transcription-coupled repair

Figure 2

The global genomic nucleotide excision repair (ggNER) detected using alkaline electrophoresis. A: Representative alkaline electrophoresis pattern in the ggNER assay of DNA-PKcs depleted HeLa-H1 cells and control HeLa-NC cells. Cells were collected at 0, 2, 4, 8 and 24 h after 10 J/m2 UV radiation, then genomic DNA was digested with T4 Endo V, and ggNER was assayed using alkaline electrophoresis. Genomic DNA without T4 Endo V treatment at the same time point was used as the control. B: A representative alkaline electrophoresis pattern in the ggNER assay of CSB depleted HeLasiRNA-107 cells and control HeLa-NC cells. HeLasiRNA-107 cells were generated from HeLa cells by stably transfecting with a vector expressing siRNA specifically targeting the CSB gene. C: A representative alkaline electrophoresis pattern in the ggNER assay of HL60 cells.

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