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Table 3 Effect of reverse transcriptase inhibitors on ORFeus-M m retrotransposition.

From: Effect of reverse transcriptase inhibitors on LINE-1 and Ty1 reverse transcriptase activities and on LINE-1 retrotransposition

  RT inhibitors Relative retrotransposition efficiencya
   5 μM 50 μM 200 μM 400 μM
NRTI AZT 0.75 ± 0.08 0.16 ± 0.02   
  d4Tb 0.28 ± 0.05 0.01 ± 0.02   
  ddC <0.0025c <0.0025c   
  3TC 0.21 ± 0.07 0.08 ± 0.01   
  ddI <0.0025c <0.0025c   
  ABC 0.80 ± 0.05 0.15 ± 0.03   
  FTC 0.34 ± 0.01 0.26 ± 0.04   
NtRTI Bis-POM PMPAb <0.0025c <0.0025c   
NNRTI NVPb 0.72 ± 0.05 0.66 ± 0.06 1.12 ± 0.08 0.79 ± 0.05
  DLVb 0.60 ± 0.05 0.02 ± 0.01   
  1. a Transposition efficiency of control assay without inhibitors was considered as 1.0. Transposition efficiency in the presence of inhibitors was indicated as relative efficiency with respect to the control. All data were normalized to cell viability with the treatment of the same amount of inhibitors. Data are mean of six independent experiments ± standard error.
  2. b Transposition efficiency was compared to the control containing the same amount of DMSO
  3. c No colonies when 1×104 cells were plated
  4. d Transposition efficiency could not be detected due to excessive cell death