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Figure 1 | BMC Biochemistry

Figure 1

From: Bergamot (Citrus bergamia Risso) fruit extracts and identified components alter expression of interleukin 8 gene in cystic fibrosis bronchial epithelial cell lines

Figure 1

Effects of bergamot extracts on IL-8 mRNA accumulation. IB3-1 were treated with ng/ml TNF-α in the presence of increasing concentrations of bergamot extracts for 24 hours, RNA was extracted and RT-PCR performed with primers specific for IL-8 and β-actin RNA sequences. A. Representative quantitative RT-PCR analysis. Triangles: RNA from untreated IB3-1 cells; squares: TNF-α treated IB3-1 cells; crosses: IB3-1 cells treated with IC50 extract 3. B: Effects of bergamot extracts on IL-8 mRNA accumulation with respect to β-actin transcripts; (a): extracts added at IC25; (b): extracts added at IC50. Results represent the average ± S.D. of three independent experiments. For each experiment triplicate RT-PCR determinations were performed. (*) p < 0.05.

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BMC Biochemistry

ISSN: 1471-2091

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