Figure 5From: Interaction of ATP with fibroblast growth factor 2: biochemical characterization and consequence for growth factor stabilityStabilization of FGF2 by ATP/Mg2+-complex. FGF2 (5 μM) in Tris-HCl (pH 7.5) was incubated with or without ATP (100 μM), MgCl2 (100 μM) and trypsin (50 ng) at 37°C for 3 h. The samples were separated by SDS-PAGE and protein was visualized by silver-staining.Back to article page