Figure 3From: Interaction of ATP with fibroblast growth factor 2: biochemical characterization and consequence for growth factor stabilityStability of FGF2 in aqueous solution. FGF2 (5 ng/mL) was incubated in 25 mM Tris, HCl (pH 7.5) at 37°C with or without preincubation with ATP (200 μM) or heparin (10 μg/mL), respectively. Samples were collected at different points of time and FGF2-concentration was determined with Human bFGF ELISA Kit (RayBiotech, USA) in triplicate.Back to article page