Figure 1From: Glycoproteomic characterization of carriers of the CD15/Lewisx epitope on Hodgkin's Reed-Sternberg cellsAnalysis of GalMBP ligands from L-428 cells with anti-Lewisx antibodies. Elution fractions from solubilized L-428 cells purified over a column of immobilized GalMBP were analyzed on 15% SDS-polyacrylamide gels followed by Coomassie blue staining or electroblotting onto nitrocellulose and probing with anti-Lewisx antibodies and radiolabelled GalMBP. The different relative intensities of some of the bands suggest that GalMBP and the antibodies bind differentially to glycans.Back to article page