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Table 4 Summary of binding kinetics measured using Octet RED96

From: The Peroxisomal Targeting Signal 1 in sterol carrier protein 2 is autonomous and essential for receptor recognition

Immobilised

biotinylated Pex5p(C)

Ligand

Association rate, kon

(1/Ms)

Dissociation rate, koff

(1/s)

Wild-type

mSCP2

2.3 × 104 (± 2.0 × 102)

5.5 × 10-5 (± 2.0 × 10-6)

Wild-type

preSCP2

3.5 × 104 (± 2.8 × 102)

7.9 × 10-5 (± 1.5 × 10-6)

S600W

mSCP2

6.5 × 103 (± 3.9 × 101)

6.0 × 10-5 (± 1.7 × 10-6)

S600W

preSCP2

7.3 × 103 (± 4.8 × 101)

5.1 × 10-5 (± 1.8 × 10-6)

R608W

mSCP2

7.2 × 103 (± 4.8 × 101)

7.3 × 10-5 (± 1.8 × 10-6)

R608W

preSCP2

9.0 × 103 (± 6.4 × 101)

5.0 × 10-5 (± 1.9 × 10-6)

D624W

mSCP2

1.1 × 104 (± 8.3 × 101)

1.5 × 10-4 (± 2.3 × 10-6)

D624W

preSCP2

1.3 × 104 (± 1.1 × 102)

8.6 × 10-5 (± 2.2 × 10-6)