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Table 4 Summary of binding kinetics measured using Octet RED96

From: The Peroxisomal Targeting Signal 1 in sterol carrier protein 2 is autonomous and essential for receptor recognition

Immobilised
biotinylated Pex5p(C)
Ligand Association rate, kon
(1/Ms)
Dissociation rate, koff
(1/s)
Wild-type mSCP2 2.3 × 104 (± 2.0 × 102) 5.5 × 10-5 (± 2.0 × 10-6)
Wild-type preSCP2 3.5 × 104 (± 2.8 × 102) 7.9 × 10-5 (± 1.5 × 10-6)
S600W mSCP2 6.5 × 103 (± 3.9 × 101) 6.0 × 10-5 (± 1.7 × 10-6)
S600W preSCP2 7.3 × 103 (± 4.8 × 101) 5.1 × 10-5 (± 1.8 × 10-6)
R608W mSCP2 7.2 × 103 (± 4.8 × 101) 7.3 × 10-5 (± 1.8 × 10-6)
R608W preSCP2 9.0 × 103 (± 6.4 × 101) 5.0 × 10-5 (± 1.9 × 10-6)
D624W mSCP2 1.1 × 104 (± 8.3 × 101) 1.5 × 10-4 (± 2.3 × 10-6)
D624W preSCP2 1.3 × 104 (± 1.1 × 102) 8.6 × 10-5 (± 2.2 × 10-6)