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Table 3 Summary of secondary structure estimations from CD spectropolarimetry

From: The Peroxisomal Targeting Signal 1 in sterol carrier protein 2 is autonomous and essential for receptor recognition

Pex5p(C) sample

% α-helix a

% β-strand

% other

NRMSD c

Wild-type

60.3 ± 1.5 b

7.0 ± 1.0

32.6 ± 0.6

0.012 ± 0.002

R608W

57.0 ± 1.0

9.0 ± 1.0

34.0 ± 0.6

0.011 ± 0.002

D624W

48.7 ± 1.5

9.0 ± 1.0

42.3 ± 0.6

0.017 ± 0.002

  1. aSecondary structure estimates derived from DICHROWEB [17] (see Methods)
  2. bMean and standard deviation over three measurements are given
  3. cNormalised root mean square deviation calculated in DICHROWEB [17]
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BMC Biochemistry

ISSN: 1471-2091

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