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Table 2 The melting temperatures of the different expressed constructs measured by differential scanning calorimetry.

From: Aglycone specificity of Thermotoga neapolitana β-glucosidase 1A modified by mutagenesis, leading to increased catalytic efficiency in quercetin-3-glucoside hydrolysis

Enzyme

Tm (°C)

ΔTm (°C)

wild-type

101.9

 

N221S/P342L

100.5

-1.4

N221S/P165L/M278I

95.5

-6.4

F219L/K214R

101.8

-0.1

F219L/P165L/M278I

93.4

-8.5

G222Q/V203M/K214R

96.8

-5.1

G222Q/P165L/M278I

94.8

-7.3

G222M

101.2

-0.7

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BMC Biochemistry

ISSN: 1471-2091

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