Primer | Direction | Mutation | Sequencea, b |
---|---|---|---|
1 | Forward | N/A | 5'-TATTCTTATCATATG AAAAAGTTTCCCGAAGGGTTC |
2 | Reverse | N/A | 5'-TATTCTTATCTCGAG ATCTGTTAGTCCGTTGTTTTTG |
3 | Forward | N/A | 5'-AATACGACTCACTATAGG |
4 | Reverse | N/A | 5'-CTAGTTATTGCTCAGCGG |
5 | Forward | F219L | 5'GACGGAAAGATAGGGATTGTTTTA AACAACGGATACTTCGA |
6 | Forward | N221S | 5'-GGGATTGTTTTCAACAGC GGATACTTCGAACCTGC |
7 | Forward | G222M | 5'-GATTGTTTTCAACAACATG TACTTCGAACCTGCAAG |
8 | Forward | G222Q | 5'- GGATTGTTTTCAACAACCAA TACTTCGAACCTGCAAGTGAGAGAG |
9 | Reverse | F219L | 5'- GACGGAAAGATAGGGATTGTTTTA AACAACGGATACTTCGA |
10 | Reverse | N221S | 5'- GCAGGTTCGAAGTATCCGCT GTTGAAAACAATCCC |
11 | Reverse | G222M | 5'-CTCTCTCACTTGCAGTAC CGAAGTATTGGTTGTTGAA |
12 | Reverse | G222Q | 5'- CTCTCTCACTTGCAGGTT CGAAGTATTGGTTGTTGAAAACAATCC |